Title

Tailed Forisomes of Canavalia gladiata: A New Model to Study Ca2+-driven Protein Contractility

Document Type

Article

Publication Date

2007

Publication Source

Annals of Botany

Volume

100

Issue

1

Inclusive pages

101-109

ISBN/ISSN

03057364

Peer Reviewed

yes

Abstract

Background and Aims Forisomes are Ca2+-dependent contractile protein bodies that form reversible plugs in sieve tubes of faboid legumes. Previous work employed Vicia faba forisomes, a not entirely unproblematic experimental system. The aim of this study was to seek to establish a superior model to study these intriguing actuators.

Methods Existing isolation procedures were modified to study the exceptionally large, tailed forisomes of Canavalia gladiata by differential interference contrast microscopy in vitro. To analyse contraction/expansion kinetics quantitatively, a geometric model was devised which enabled the computation of time-courses of derived parameters such as forisome volume from simple parameters readily determined on micrographs.

Key Results Advantages of C. gladiata over previously utilized species include the enormous size of its forisomes (up to 55 µm long), the presence of tails which facilitate micromanipulation of individual forisomes, and the possibility of collecting material repeatedly from these fast-growing vines without sacrificing the plants. The main bodies of isolated Canavalia forisomes were box-shaped with square cross-sections and basically retained this shape in all stages of contraction. Ca2+-induced a 6-fold volume increase within about 10–15 s; the reverse reaction following Ca2+-depletion proceeded in a fraction of that time.

Conclusions The sword bean C. gladiata provides a superior experimental system which will prove indispensable in physiological, biophysical, ultrastructural and molecular studies on the unique ATP-independent contractility of forisomes.

Keywords

forisomes, botany

Disciplines

Medical Sciences

 
 

Link to Original Published Item

http://dx.doi.org/10.1093/aob/mcm080