Document Type

Article

Publication Date

2013

Publication Source

Plant Physiology and Biochemistry

Volume

70

Inclusive pages

52-60

Publisher

Elsevier

ISBN/ISSN

0981-9428

Peer Reviewed

yes

Abstract

The single cell alga Chlamydomonas reinhardtii is capable of importing purines as nitrogen sources. An analysis of the annotated C. reinhardtii genome reveals at least three distinct gene families encoding for known nucleobase transporters. In this study the solute transport and binding properties for the lone C. reinhardtii nucleobase cation symporter 1 (CrNCS1) are determined through heterologous expression in Saccharomyces cerevisiae. CrNCS1 acts as a transporter of adenine, guanine, uracil and allantoin, sharing similar e but not identical e solute recognition specificity with the evolutionary distant NCS1 from Arabidopsis thaliana. The results suggest that the solute specificity for plant NCS1 occurred early in plant evolution and are distinct from solute transport specificities of single cell fungal NCS1 proteins.

Keywords

Nucleobase cation symporter 1, Purine, Pyrimidine, Chlamydomonas, Transport

Disciplines

Biology

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Link to Original Published Item

http://dx.doi.org/10.1016/j.plaphy.2013.05.015