Title

Metabolic Studies of Pyridoxamine (Vitamin B-6) Metabolism in Rats

Document Type

Presentation

Presentation Date

4-10-2011

Conference Name

Experimental Biology 2011

Conference Location

Washington, DC

Source of Publication

FASEB Journal

Publisher

Federation of American Societies for Experimental Biology

Place of Publication

Bethesda, MD

Publication Date

2011

Volume

25

Inclusive pages

608.3

Peer Review

Contributed

Abstract

Pyridoxamine (PM), a metabolic isomer of vitamin B6, has been studied as an agent to reduce/prevent diabetic complications, based upon its ability to prevent formation of advanced glycation end-products and act as an anti-oxidant (Voziyan et al., Cell. Mol. Life Sci. 62:1671,2005). As a preliminary study determining effect of PM on bone collagen matrix in diabetic rats, Sprague-Dawley rats were given subcutaneous saline (control), 50 mg/kg, or 100 mg/kg pyridoxamine; plasma and urine samples were collected over 24 hours. Samples were analyzed by HPLC to quantitate vitamers of vitamin B6 (PLP, 4PA, PL), as well as PM and pyridoxamine-5'-phosphate. Urine samples were also analyzed for 4PA, and also PM and N-methylpyridoxamine, to determine if rats, like dogs, are able to methylate pyridoxamine (Ericson et al., Bioorg.Med.Chem.Lett. 18:1845 2008). Data were analyzed using the WINSAAM modeling program, and the plasma half life of PM in rats receiving 50 mg/kg was calculated at 1.43 hours; the half-life in rats receiving 100 mg/kg was calculated to be 1.6 hrs. Supported by NIH grant R01 AR 047838 (DBB).

Keywords

vitamin B-6, pyridoxamine, diabetes

Disciplines

Chemistry | Molecular, Genetic, and Biochemical Nutrition

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