Download Full Text (320 KB)
Dr. Punya Nachappa
Department of Biology
Soybean growers in all major soybean-producing areas of North America are facing a potential new threat to soybean production due to an emerging soybean viral disease, Soybean Vein Necrosis Virus (SVNV). Currently the disease has been detected in 16 states in the Mid-west including Indiana. The disease can cause up to 46% yield loss in soybean. The virus is transmitted from plant-to-plant by an insect vector, soybean thrips (Neohydathothrips variablilis). Soybean thrips acquire SVNV in the early larval stage and transmit the virus to the soybean plants as adults. Like other plant feeding insects, soybean thrips have endosymbiotic bacteria that influence fundamental biological processes such as synthesis of essential nutrients and recycling of nitrogenous wastes. Moreover, transmission of some pathogens may be affected by endosymbiotic bacteria. There is no information on the diversity of endosymbionts in soybean thrips. Hence, the objective of our study was to determine the diversity of endosymbiotic bacteria in field-collected soybean thrips. We performed DNA extraction from a pool of 100 adult male and female soybean thrips. The DNA was then subjected to 16S rDNA pyrosequencing to determine the endosymbiont bacteria in the insect sample. Our results indicated a diversity of bacteria in soybean thrips including, Pseudomonas sp, S treptomyces sp, Herbaspirillum sp and Acinetobacter sp. Pseudomonas sp accounted for nearly 50% of the bacterial diversity in the sample. This is the first comprehensive survey of bacteria associated with soybean thrips gut. Results of this study will provide a foundation for future studies to elucidate the roles of gut microbes in SVNV transmission.
Kim, Min Jung, "16S rDNA Pyrosequencing Indicates Pseudomonas sp as the Predominant Bacteria in Gut Microbiome of Soybean Thrips, Neohydathothrips variablilis" (2016). 2016 IPFW Student Research and Creative Endeavor Symposium. 36.