The nucleobase cation symporter 1 of Chlamydomonas reinhardtii and that of the evolutionarily distant Arabidopsis thaliana display parallel function and establish a plant-speciﬁc solute transport proﬁle
Plant Physiology and Biochemistry
The single cell alga Chlamydomonas reinhardtii is capable of importing purines as nitrogen sources. An analysis of the annotated C. reinhardtii genome reveals at least three distinct gene families encoding for known nucleobase transporters. In this study the solute transport and binding properties for the lone C. reinhardtii nucleobase cation symporter 1 (CrNCS1) are determined through heterologous expression in Saccharomyces cerevisiae. CrNCS1 acts as a transporter of adenine, guanine, uracil and allantoin, sharing similar e but not identical e solute recognition speciﬁcity with the evolutionary distant NCS1 from Arabidopsis thaliana. The results suggest that the solute speciﬁcity for plant NCS1 occurred early in plant evolution and are distinct from solute transport speciﬁcities of single cell fungal NCS1 proteins.
Nucleobase cation symporter 1, Purine, Pyrimidine, Chlamydomonas, Transport
Jessica R. Schein, Kevin A. Hunt, Janet A. Minton, Neil P. Schultes, and George Mourad (2013).
The nucleobase cation symporter 1 of Chlamydomonas reinhardtii and that of the evolutionarily distant Arabidopsis thaliana display parallel function and establish a plant-speciﬁc solute transport proﬁle. Plant Physiology and Biochemistry.70, 52-60. Elsevier.
Available for download on Saturday, January 01, 2050