Document Type
Article
Publication Date
3-2016
Publication Source
Plant Physiology and Biochemistry
Volume
100
Inclusive pages
12-17
DOI
doi:10.1016/j.plaphy.2015.12.014
Publisher
Elsevier
ISBN/ISSN
0981-9428
Peer Reviewed
yes
Abstract
As part of an evolution-function analysis, two nucleobase cation symporter 1 (NCS1) from the moss Physcomitrella patens (PpNCS1A and PpNCS1B) are examined – the first such analysis of nucleobase transporters from early land plants. The solute specificity profiles for the moss NCS1 were determined through heterologous expression, growth and radiolabeled uptake experiments in NCS1-deficient Saccharomyces cerevisiae. Both PpNCS1A and 1B, share the same profiles as high affinity transporters of adenine and transport uracil, guanine, 8-azaguanine, 8-azaadenine, cytosine, 5-fluorocytosine, hypoxanthine, and xanthine. Despite sharing the same solute specificity profile, PpNCS1A and PpNCS1B move nucleobase compounds with different efficiencies. The broad nucleobase transport profile of PpNCS1A and 1B differs from the recently-characterized Viridiplantae NCS1 in breadth, revealing a flexibility in solute interactions with NCS1 across plant evolution.
Keywords
Physcomitrella patens, Nucleobase cation symporter 1, Transport, Purine and pyrimidine
Disciplines
Biology
Opus Citation
Janet Minton, Micah Rapp, Amanda Stoffer, Neil P. Schultes, and George Mourad (2016).
Heterologous Complementation Studies Reveal the Solute Transport Profiles of a Two-Member Nucleobase Cation Symporter 1 (NCS1) Family in Physcomitrella Patens. Plant Physiology and Biochemistry.100, 12-17. Elsevier.
https://opus.ipfw.edu/biology_facpubs/289