Department of Biology
Indiana University – Purdue University Fort Wayne
IPFW Sigma Xi (Scientific Research Honor Society) Student Research Competition
Mutant viruses containing deletions in DNA encapsidation genes have been characterized in detail for Herpes simplex type I, and to some extent for Pseudorabies virus, and Human cytomegalovirus. However, similar mutants for Varicella-zoster virus do not exist. It is expected that the deletion of each putative DNA encapsidation gene will yield a phenotype consistent with a defect in DNA cleavage and/or packaging. These mutant viruses will be isolated by site directed homologous recombination in complementing cell lines expressing the respective DNA encapsidation proteins.
Pearl C. Pfiester (2010).
Construction and Characterization of Varicella-zoster virus DNA Encapsidation Protein Expressing Cell Lines.