2015 IPFW Student Research and Creative Endeavor Symposium



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Faculty Sponsor

Dr. Punya Nachappa


Department of Biology

University Affiliation

Indiana University – Purdue University Fort Wayne


Soybean growers are facing a potential new threat to production due to an emerging viral disease, Soybean Vein Necrosis Virus (SVNV). Since its initial discovery in soybean fields in Tennessee in 2008, the disease has rapidly spread to 16 states in the U.S. including Indiana. Although it is widely-accepted that thrips are vectors of SVNV, it is not known which of the many species found in soybean fields such as soybean thrips, flower thrips and tobacco thrips transmits the virus. Because SVNV is newly described, there is no information about the economic impact of the disease and management options. To develop sustainable management practices for SVNV, a sound understanding of the basic aspects of the relationship between the plant, virus, and the insect vector is essential. The objectives of our study were to 1) Determine the time required for acquisition of SVNV molecules also called Acquisition Access Period (AAP) by soybean thrips and tobacco thrips, and 2) Determine the time required for successful transmission of SVNV to a healthy plant also termed Inoculation Access Period (IAP). First instar nymphs of soybean thrips and tobacco thrips were exposed to SVNV-infected leaves for 6, 12 and 24 hours for acquisition of virus (AAP). Once the nymphs developed to adults they were allowed 24, 48, and 72 hours of inoculation of virus to healthy leaves (IAP). Our results suggest that soybean thrips and tobacco thrips differed in their ability to acquire virus. Soybean thrips were more efficient in transmitting the virus to healthy leaves. Gene expression analysis confirmed different levels of virus in soybean and tobacco thrips. These data provide new information about the factors affecting competencies in vector and non-vector species.


Biology | Life Sciences

Analysis of competence in two potential insect vectors of Soybean vein necrosis virus

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